LongTREC presents:
Toward the use of nanopore RNA sequencing technologies in the clinic: challenges and opportunities
Xanthi-Lida Katopodi, Oguzhan Begik, Eva Maria Novoa
RNA molecules are increasingly seen as potential clinical biomarkers: signals in our body that can help detect or monitor disease. Today, RNA is usually measured with short-read sequencing or PCR tests. These methods count how much RNA there is, but they miss important details, such as chemical modifications that can change how RNA behaves and are often altered in disease.
Direct RNA sequencing from Oxford Nanopore reads native RNA molecules, capturing both how abundant they are and their modifications at the same time. In this review, the authors discuss the challenges still to be overcome and the emerging opportunities for using this technology in the clinic. They build on its rapid technical progress and on growing evidence of altered RNA in body fluids.
By mapping the path from the lab to the clinic, the review highlights how nanopore RNA sequencing could transform personalized medicine and disease monitoring, and what is needed to get there.
Read the full text here: https://doi.org/10.1093/nar/gkaf128
