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LongTREC

Isoform Identification and Quantification with IsoQuant

In today’s BioBam article, Fabian Jetzinger, Doctoral Candidate, introduces IsoQuant, a powerful tool for isoform identification and quantification in long-read transcriptomics.
 
IsoQuant sets itself apart from traditional tools through its unique graph-based strategy, which constructs an intron-graph to define complex transcript models from PacBio or ONT data.
 
Key Benefits of IsoQuant in OmicsBox:
  • Discovery Mode: Identify novel transcripts even without a reference annotation or supporting short read.
  • Reference-Based Quantification: Enhance efficiency by quantifying genes and transcripts directly from high-quality existing annotations.
  • Seamless Integration: Transition instantly from identification to downstream analysis, including structural categorization with SQANTI3 and differential expression with edgeR or NOISeq.

Experience a streamlined, user-friendly workflow that moves from raw long-read data to biological insights within a single interface.

📖 Read the full article here: https://www.biobam.com/isoquant-long-read-isoform-identification-and-quantification/

:tv: Watch the full tutorial here: https://www.youtube.com/watch?v=EClq3l2KR1A