Research Project 4
Long read RNA sequencing to annotate the genomes across the eukaryotic tree of life
About the Project
Centre for Genomic Regulation
Barcelona, Spain
Roderic Guigó
Main Supervisor
Universitat Pompeu Fabra
PhD enrolment
Yearly salary
29.959,57 € (Gross)
Research Objectives
- Develop methods to efficiently map lrRNA-seq reads across species.
- Develop methods to normalize lrRNA-seq libraries and/or to target low abundant transcripts.
- Develop methods for targeting and sequencing very long RNA molecules.
Envisioned Secondments
- Wobble Genomics (Richard Kuo): Learn company’s library normalization methods for lrRNA-se.
- SU (Kristoffer Sahlin): Assess SU’s mapping algorithms for long transcripts and across-species mapping.
About the Main Supervisor and Host Group
Roderic Guigó
Centre for Genomic Regulation,
Spain
The overarching theme of the research in Roderic Guigo’s lab at CRG is the understanding of the information encoding in genomic sequences, and how this information is processed in the pathway leading from DNA to protein sequences. More specifically, we are interested in the epigenetic regulation of gene expression and RNA processing, the relationship between molecular phenotypes and higher order endophenotypes and organismal phenotypes, and the identification of functional regions on the genome of all living beings. Our group is mostly computational, and we do both large-scale data analysis and development of methods, but it has also an important experimental component. We have participated and participate in many large scale international functional genomics projects, such as ENCODE, GTEx, BluePrint and others.